Ganoderma
Nitroblue Tetrazolium (NBT) Dye Test In Infectious Disease In Children
Mahidol University Annual Research Abstracts
Faculty of Ganoderma Medicine Ramathibodi Hospital
Cose tolerance test were performed in all cases.Blood levels
for fasting cholesterol and triglyceride were drawn in 29 children.
Using WHO criteria, our results show that 12 children (12/39:31%)
have impaired glucose tolerance test, 7 children (7/39:18%) have
diabetes, 15 children (15/29:49%)have cholesterol levels over 200
mg/dl and 22children (22/29: 76%) have at least one risk factors
(either abnormal GTT and high cholesterol). None of these children
has polyurea or weight loss.
We
conclude that all obese children who have family history of type II
diabetes and acanthosis nigrican should be screened for cholesterol
levels and diabetes.
Since adult endocrinologists take care of
their parents, awareness of this problem should be raised as an
issue for prevention of type II diabetes in children
Nitroblue Tetrazolium (NBT) Dye Test in Infectious
Disease in Children (No. 166) Dussadee
Charoenpipop1., and Somsak Lolekha2
Division of Pediatric Infectious Diseases,
1Department of Research Center, 2Department
of Pediatrics, Faculty of medicine, Ramathibodi Hospital, Mahidol
University, Bangkok, Thailand. Key words :
Nitroblue Tetrazodium Dye, Infectious Disease
NBT
dye test is used for the detection of the metabolic activities of
Polymorphonuclear leukocyte (PMN) which relates with the
intracellular bacterial killing activity.
The test composed of two
conditions. They are stimulated and non-stimulated NBT test.
Stimulated NBT test is used for the detection of the dysfunction of
PMN. Non stimulated NBT test is used for the detection of bacterial
infection.
After infection, organisms are phagocytized by PMN and
the metabolic steps occurred during intracellular killing of
ingested organism are sufficient to cause spontaneous in vitro
reduction of the colorless soluble NBT dye to produce an insoluble
blue black formazan precipitate. In normal childrens, the value of
non-stimulated NBT positive should be in 0-18 % and rise to 30-60 %
of range after stimulation test. But in infectious status, the
value of non-stimulated NBT positive will rise to 25-50 %. If it is
still in 0-18 % of range, it indicates that phagocytosis system of
the host is not functioned normally.
In 70 NBT test results from
children patients with various kind of infectious diseases; 20
cases were PMN dysfunction, 18 cases were not bacterial infections
and 32 cases were bacterial infections. It is found that besides
its simplicity, rapidity and economy, the NBT test is still able to
be used as a tool for evaluation of PMN leukocyte function and for
separating bacterial infection from viral infection which was
clinically useful for further treatment.
DIFFERENT RETICULOCYTE VOLUME IN DIABETES MELLITUS
PATIENTS
WITH AND WITHOUT HYPERCHOLESTEROLEMIA AND/OR
HYPERTRIGLYCERIDEMIA (NO. 167) Porntip
Bunyaratvej1, Surat Komindr2, Raewadee
Wised-panichkij3 1Research
Center, 2 Department of Medicine, 3Blood
Disease Diagnosis Center, Sirikit Medical Center, Department of
Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol
University, Bangkok 10400, Thailand.
Key words :
Disbetes Mellitus, Hypercholesterolemia, Hypertrigly-ceridemia,
Reticulocyte
Red
cells and reticulocytes from patients with diabetes mellitus (DM)
were analysed using laser and computer technology of H*3 hematology
analyzer. Four groups of diabetes mellitus patients: diabetes with
normolipemia (DM) (n=12) and DM patients with excess triglyceride
(DM-T) (n=7) or cholesterol (DM-C) (n=21) or both (DM-TC) (n=21)
were studied. Mean corpuscular volume of mature red cells (Mean+SD
= 93.6+5.
5 fl) and reticulocyte (119.1+12.3 f1) of patients with
DM-DT was not significantly increased from normal (red cell,
90.0+3.
5 f1; reticulocyte, 115.2+7.3 f1). Plasma triglyceride
levels had no significant correlation with red cell MCV,
reticulocyte MCV and %Hb Alc.
This suggests that high triglyceride
levels in DM are not dose-dependent in producing increased MCV of
red cells and reticulocytes. Comparing between DM-T and DM-C, red
cells and reticulocytes from DM-C patients had significantly
decreased MCV (red cell, 85.5+6.1 fL; reticulocyte, 103.
8+7.4 fL).
Plasma cholesterol levels were inversely correlated with MCV of red
cells (r=-0.377, p=0.
003) and also MCV of reticulocytes (r=-0.418,
p=0.001). In addition, cholesterol levels showed considerable
correlation with Hb Alc (r=0.
572, p=0.004). The red cell volume
change in DM-C patients may be partly due to the shift in balance
of cholesterol exchange between red cell membranes and serum
lipoproteins. (J Med Assoc Thai 2000;
83:790-6.
)
LEVELS OF PLASMA LIPID PEROXIDE PRODUCTS AND
ANTIOXIDANT
STATUS IN RHEUMATOID ARTHRITIS (NO. 168)
Saowanee Kajanachumpol1, Monchand
Vanichapuntu1, Oravan Verasertniyom1, Kitti
Totemchokchyakarn2 and Mongkol Vatanasuk2
1Research Center, Faculty of Medicine,
2Division of Allergy, Immunology and Rheumatology,
Department of Medicine, Ramathibodi Hospital, Mahidol University,
Bangkok, 10400, Thailand. Key words : Lipid
peroxide products, Antioxidant status, Rheumatoid Arthritis
Oxygen
free radicals have been implicated as mediators of tissue damage in
patients with rheumatoid arthritis. The aim of our study was to
assess the lipid peroxide products and antioxidant status in
rheumatoid arthritis patients (RA).
The study involved
determination of two plasma lipid peroxide products,
malondialdehyde (MDA) and conjugated dienes (CD), two plasma
antioxidant vitamins (C and E) in 91 RA patients and 26 healthy
subjects. The results showed that rheumatoid patients had increased
plasma CD but not MDA and decreased plasma vitamin E, when properly
expressed per unit cholesterol and triglyceride. This finding
suggested that RA patients had increased oxidant stress that might
play a role in the tissue damage and inflammation process of this
disease. (Southeast Asian J Trop Med Public Health
2000; 31:335-8.
)
.